Alleviation of Phytophthora infestans Mediated Necrotic Stress in the Transgenic Potato (Solanum tuberosum L.) with Enhanced Ascorbic acid Accumulation.
Identifieur interne : 000586 ( Main/Exploration ); précédent : 000585; suivant : 000587Alleviation of Phytophthora infestans Mediated Necrotic Stress in the Transgenic Potato (Solanum tuberosum L.) with Enhanced Ascorbic acid Accumulation.
Auteurs : Ill-Min Chung [Corée du Sud] ; Baskar Venkidasamy [Inde] ; Chandrama Prakash Upadhyaya [Inde] ; Gurusaravanan Packiaraj [Inde] ; Govindasamy Rajakumar [Corée du Sud] ; Muthu Thiruvengadam [Corée du Sud]Source :
- Plants (Basel, Switzerland) [ 2223-7747 ] ; 2019.
Abstract
Potato is the most widely cultivated non-cereal crop in the world, and like any other crop, it is susceptible to yield losses because of various factors, including pathogen attacks. Among the various diseases of potato, late blight caused by the oomycete Phytophthora infestans is considered as the most devastating disease worldwide. In this study, transgenic potato plants overexpressing the D-galacturonic acid reductase (GalUR) gene with an enhanced level of cellular L-ascorbate (L-AsA) were challenged with Phytophthora infestans to determine the level of stress tolerance induced in those plants. With the onset of pathogen infection, necrotic lesions progressively expanded and became necrotic in the control plants. The transgenic potato lines with enhanced ascorbic acid showed reduced necrotic lesions. Hydrogen peroxide (H2O2) and malondialdehyde (MDA) levels were relatively lower in transgenic plants compared to the untransformed control (UT) plants. The mRNA expressions of pathogenesis-related (PR) genes, such as pathogenesis related 1 (PR1) and phenylalanine ammonia-lyase (PAL) were slightly higher in GalUR overexpressing transgenic lines as compared to the untransformed control plants. Pathogen infection also altered the mRNA expression of genes associated with gibberellic acid (GA) and abscisic acid (ABA) biosynthesis. Furthermore, the increase in various antioxidant enzymes was also observed in the gene expression analysis with the transgenic plants. The complete loss of the pathogen growth and disease occurrence was not observed in our study; however, the findings indicated that an increase in the level of cellular L-ascorbate in the transgenic potato leads to enhanced cellular antioxidants, PR genes and plant defense hormones, such as GA and ABA resulting in the reduction of the disease symptoms caused by the Phytophthora infestans.
DOI: 10.3390/plants8100365
PubMed: 31547616
PubMed Central: PMC6843151
Affiliations:
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sort="Chung, Ill Min" uniqKey="Chung I" first="Ill-Min" last="Chung">Ill-Min Chung</name><affiliation wicri:level="1"><nlm:affiliation>Department of Applied Bioscience, College of Life and Environmental Sciences, Konkuk University, Seoul 05029, Korea. imcim@konkuk.ac.kr.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Applied Bioscience, College of Life and Environmental Sciences, Konkuk University, Seoul 05029</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Venkidasamy, Baskar" sort="Venkidasamy, Baskar" uniqKey="Venkidasamy B" first="Baskar" last="Venkidasamy">Baskar Venkidasamy</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Bharathiar University, Coimbatore 641046, Tamil Nadu, India. baskarbt83@gmail.com.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biotechnology, Bharathiar University, Coimbatore 641046, Tamil Nadu</wicri:regionArea><wicri:noRegion>Tamil Nadu</wicri:noRegion></affiliation></author><author><name sortKey="Upadhyaya, Chandrama Prakash" sort="Upadhyaya, Chandrama Prakash" uniqKey="Upadhyaya C" first="Chandrama Prakash" last="Upadhyaya">Chandrama Prakash Upadhyaya</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Plant Molecular Biology, Department of Biotechnology, Dr. Harisingh Gour Central University, Sagar 470003, Madhya Pradesh, India. cpupadhyay@gmail.com.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Laboratory of Plant Molecular Biology, Department of Biotechnology, Dr. Harisingh Gour Central University, Sagar 470003, Madhya Pradesh</wicri:regionArea><wicri:noRegion>Madhya Pradesh</wicri:noRegion></affiliation></author><author><name sortKey="Packiaraj, Gurusaravanan" sort="Packiaraj, Gurusaravanan" uniqKey="Packiaraj G" first="Gurusaravanan" last="Packiaraj">Gurusaravanan Packiaraj</name><affiliation wicri:level="1"><nlm:affiliation>Department of Botany, Bharathiar University, Coimbatore 641046, Tamil Nadu, India. pgurusaravanan@gmail.com.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Botany, Bharathiar University, Coimbatore 641046, Tamil Nadu</wicri:regionArea><wicri:noRegion>Tamil Nadu</wicri:noRegion></affiliation></author><author><name sortKey="Rajakumar, Govindasamy" sort="Rajakumar, Govindasamy" uniqKey="Rajakumar G" first="Govindasamy" last="Rajakumar">Govindasamy Rajakumar</name><affiliation wicri:level="1"><nlm:affiliation>Department of Applied Bioscience, College of Life and Environmental Sciences, Konkuk University, Seoul 05029, Korea. microlabsraj@gmail.com.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Applied Bioscience, College of Life and Environmental Sciences, Konkuk University, Seoul 05029</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Thiruvengadam, Muthu" sort="Thiruvengadam, Muthu" uniqKey="Thiruvengadam M" first="Muthu" last="Thiruvengadam">Muthu Thiruvengadam</name><affiliation wicri:level="1"><nlm:affiliation>Department of Applied Bioscience, College of Life and Environmental Sciences, Konkuk University, Seoul 05029, Korea. muthu@konkuk.ac.kr.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Applied Bioscience, College of Life and Environmental Sciences, Konkuk University, Seoul 05029</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author></analytic><series><title level="j">Plants (Basel, Switzerland)</title><idno type="ISSN">2223-7747</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Potato is the most widely cultivated non-cereal crop in the world, and like any other crop, it is susceptible to yield losses because of various factors, including pathogen attacks. Among the various diseases of potato, late blight caused by the oomycete <i>Phytophthora infestans</i> is considered as the most devastating disease worldwide. In this study, transgenic potato plants overexpressing the <i>D-galacturonic acid reductase</i> (<i>GalUR</i>) gene with an enhanced level of cellular L-ascorbate (L-AsA) were challenged with <i>Phytophthora infestans</i> to determine the level of stress tolerance induced in those plants. With the onset of pathogen infection, necrotic lesions progressively expanded and became necrotic in the control plants. The transgenic potato lines with enhanced ascorbic acid showed reduced necrotic lesions. Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and malondialdehyde (MDA) levels were relatively lower in transgenic plants compared to the untransformed control (UT) plants. The mRNA expressions of pathogenesis-related (<i>PR</i>) genes, such as pathogenesis related 1 (<i>PR1</i>) and phenylalanine ammonia-lyase (<i>PAL</i>) were slightly higher in <i>GalUR</i> overexpressing transgenic lines as compared to the untransformed control plants. Pathogen infection also altered the mRNA expression of genes associated with gibberellic acid (GA) and abscisic acid (ABA) biosynthesis. Furthermore, the increase in various antioxidant enzymes was also observed in the gene expression analysis with the transgenic plants. The complete loss of the pathogen growth and disease occurrence was not observed in our study; however, the findings indicated that an increase in the level of cellular L-ascorbate in the transgenic potato leads to enhanced cellular antioxidants, PR genes and plant defense hormones, such as GA and ABA resulting in the reduction of the disease symptoms caused by the <i>Phytophthora infestans</i>.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">31547616</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Print">2223-7747</ISSN><JournalIssue CitedMedium="Print"><Volume>8</Volume><Issue>10</Issue><PubDate><Year>2019</Year><Month>Sep</Month><Day>23</Day></PubDate></JournalIssue><Title>Plants (Basel, Switzerland)</Title><ISOAbbreviation>Plants (Basel)</ISOAbbreviation></Journal><ArticleTitle>Alleviation of <i>Phytophthora infestans</i> Mediated Necrotic Stress in the Transgenic Potato (<i>Solanum tuberosum</i> L.) with Enhanced Ascorbic acid Accumulation.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E365</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/plants8100365</ELocationID><Abstract><AbstractText>Potato is the most widely cultivated non-cereal crop in the world, and like any other crop, it is susceptible to yield losses because of various factors, including pathogen attacks. Among the various diseases of potato, late blight caused by the oomycete <i>Phytophthora infestans</i> is considered as the most devastating disease worldwide. In this study, transgenic potato plants overexpressing the <i>D-galacturonic acid reductase</i> (<i>GalUR</i>) gene with an enhanced level of cellular L-ascorbate (L-AsA) were challenged with <i>Phytophthora infestans</i> to determine the level of stress tolerance induced in those plants. With the onset of pathogen infection, necrotic lesions progressively expanded and became necrotic in the control plants. The transgenic potato lines with enhanced ascorbic acid showed reduced necrotic lesions. Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and malondialdehyde (MDA) levels were relatively lower in transgenic plants compared to the untransformed control (UT) plants. The mRNA expressions of pathogenesis-related (<i>PR</i>) genes, such as pathogenesis related 1 (<i>PR1</i>) and phenylalanine ammonia-lyase (<i>PAL</i>) were slightly higher in <i>GalUR</i> overexpressing transgenic lines as compared to the untransformed control plants. Pathogen infection also altered the mRNA expression of genes associated with gibberellic acid (GA) and abscisic acid (ABA) biosynthesis. Furthermore, the increase in various antioxidant enzymes was also observed in the gene expression analysis with the transgenic plants. The complete loss of the pathogen growth and disease occurrence was not observed in our study; however, the findings indicated that an increase in the level of cellular L-ascorbate in the transgenic potato leads to enhanced cellular antioxidants, PR genes and plant defense hormones, such as GA and ABA resulting in the reduction of the disease symptoms caused by the <i>Phytophthora infestans</i>.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chung</LastName><ForeName>Ill-Min</ForeName><Initials>IM</Initials><AffiliationInfo><Affiliation>Department of Applied Bioscience, College of Life and Environmental Sciences, Konkuk University, Seoul 05029, Korea. imcim@konkuk.ac.kr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Venkidasamy</LastName><ForeName>Baskar</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, Bharathiar University, Coimbatore 641046, Tamil Nadu, India. baskarbt83@gmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Upadhyaya</LastName><ForeName>Chandrama Prakash</ForeName><Initials>CP</Initials><AffiliationInfo><Affiliation>Laboratory of Plant Molecular Biology, Department of Biotechnology, Dr. Harisingh Gour Central University, Sagar 470003, Madhya Pradesh, India. cpupadhyay@gmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Packiaraj</LastName><ForeName>Gurusaravanan</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Department of Botany, Bharathiar University, Coimbatore 641046, Tamil Nadu, India. pgurusaravanan@gmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rajakumar</LastName><ForeName>Govindasamy</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Department of Applied Bioscience, College of Life and Environmental Sciences, Konkuk University, Seoul 05029, Korea. microlabsraj@gmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Thiruvengadam</LastName><ForeName>Muthu</ForeName><Initials>M</Initials><Identifier Source="ORCID">0000-0003-0986-5484</Identifier><AffiliationInfo><Affiliation>Department of Applied Bioscience, College of Life and Environmental Sciences, Konkuk University, Seoul 05029, Korea. muthu@konkuk.ac.kr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>09</Month><Day>23</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Plants (Basel)</MedlineTA><NlmUniqueID>101596181</NlmUniqueID><ISSNLinking>2223-7747</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Phytophthora infestans</Keyword><Keyword MajorTopicYN="N">Solanum tuberosum</Keyword><Keyword MajorTopicYN="N">ascorbic acid</Keyword><Keyword MajorTopicYN="N">reactive oxygen species</Keyword><Keyword MajorTopicYN="N">transgenic plants</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>06</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>09</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>09</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>9</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>9</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>9</Month><Day>25</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId 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